LEE E. HUGHES
2220 Lattimore St.
Denton, TX 76201-4700
(940) 380-0150
Lhughes@jove.acs.unt.edu

EDUCATION

University of North Texas, Denton, Texas. Ph.D in Microbiology, May, 1998.
Dissertation Title: "Pyrimidine Biosynthesis in the Genus Streptomyces: Characterization of Aspartate Transcarbamoylase and Its Interactions with Other Pyrimidine Enzymes."
Advisor: Dr. Gerard A. O'Donovan

University of North Texas, Denton, Texas. M.S. in Biology, August, 1994.

University of North Texas, Denton, Texas. B.A. (University Honors), May, 1987. Major: English, Minor: Spanish.

LABORATORY EXPERIENCE

Bacterial culture and maintenance of stock cultures, chromosomal and plasmid DNA isolation, polyacrylamide and agarose gel electrophoresis, enzyme assays, protein purification, high performance liquid chromatography, size-exclusion chromatography, electron microscopy, DNA cloning and sequencing, polymerase chain reaction.

Lecturer, University of Texas at Dallas, Richardson, TX. Taught integrated lecture and laboratory for upper-level general microbiology course. Developed lower-level microbiology course with laboratory for non-science majors. Supervised laboratory preparation and teaching assistants.  Prepared lecture material for Powerpoint presentations and developed a Web site for lecture notes. (January 1999 - present)

Adjunct Faculty, University of North Texas, Denton, TX. Taught lecture in a team-taught introductory biology course for science majors. Taught sophomore-level general microbiology course and supervised laboratory instruction. Taught upper-level course in genetics. (August 1998 - present)

Adjunct Faculty, Brookhaven College, Dallas County Community College District, Farmers Branch, TX. Taught lecture and laboratory section of microbiology course to general science and allied health majors. (June 1998 - present)

Associate Faculty, Collin County Community College District, McKinney, TX. Taught laboratory section of Anatomy and Physiology I. (January 2000 - present)

Laboratory Coordinator and Teaching Assistant, Biological Sciences, University of North Texas, Denton, TX. Taught laboratory sections for Animal and Human Biology, Principles of Biology II, Biology of Microorganisms, Food Microbiology, General Bacteriology, Elementary Biochemistry, and Biochemistry and Molecular Biology of the Gene. Since 1995, coordinated teaching assistants in multiple sections of the Biology of Microorganisms laboratory. Organized laboratory schedule and teaching materials. Ordered supplies and oversaw the preparation of laboratory media and cultures. (September 1991 - May 1998)

Assistant Director of Admissions, Admissions Office, University of North Texas, Denton, TX. Responsible for recruitment of underrepresented students, maintenance of admissions statistical data, and supervision of freshman orientation reservations staff. Provided information to prospective students and parents. Interviewed students and made admissions decision recommendations. (September 1987 - August 1991)

Seminars/Presentations

Virtual unknowns on the World-Wide Web. L.E. Hughes. Texas Branch - ASM Meeting, Fort Worth, TX. November, 1999.

Interactions between enzymes of the pyrimidine biosynthetic pathway in Streptomyces griseus. L.E. Hughes and G.A. O'Donovan. Texas Branch - ASM Meeting, San Antonio, TX. December, 1996.

Metabolismo de pirimidinas. Invited seminar. Universidad Autonoma Chihuahua, Chihuahua, Chih., Mexico. March, 1995.

Microbial Diversity at Woods Hole (with V.G. Nevarez-Moorillon). University of North Texas Biological Sciences Seminar Series, Denton, TX. September, 1993.

Hughes, L.E., Hooshdaran, M.Z., and G.A. O'Donovan. 1999. Streptomyces aspartate transcarbamoylase is a dodecamer with dihydroorotase activity. Current Microbiology 39, 175-179.

Hughes, L.E., Beck, D.E., and G.A. O'Donovan. (in preparation). Pathways of pyrimidine salvage in Streptomyces.

Hooten, J.J., Hughes, L.E., and G.A. O'Donovan. (in preparation). Identification and characterization of the pyrimidine biosynthetic operon in Streptomyces.

Beck, D.E., Hughes, L.E., and G.A. O'Donovan. 1998. Microorganisms: A Laboratory Manual. Kendall/Hunt Publishing Co., Dubuque, IA.

Hughes, L.E. 1999. Internet virtual unknowns: a simple method for preparing a customized practice unknown onthe world-wide web, abstr. W-21, p. 668. In Abstracts of the 99th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Hughes, L.E., Hooshdaran, M., and G.A. O'Donovan. 1998. Characterization of subunit structure of the aspartate transcarbamoylase/dihydroorotase complex from Streptomyces griseus, abstr. K-34, p. 332. In Abstracts of the 98th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Hooten, J.J., Hughes, L.E., Murphy, J.L., and G.A. O'Donovan. 1998. Isolation and cloning of pyrimidine genes from Streptomyces, abstr. H-166, p. 304. In Abstracts of the 98th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Hughes, L.E., Patel, M.V., and G.A. O'Donovan. 1997. Co-purification of aspartate transcarbamoylase and dihydroorotase in Streptomyces: A CAD-like enzyme in a prokaryote?, abstr. K-57, p. 351. In Abstracts of the 97th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Hughes, L.E., Horton, M.G., and C. Ingle. 1996. Regulation of pyrimidine biosynthesis in Streptomyces griseus, abstr. K-161, p. 208. In Abstracts of the 96th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Linscott, A.J., Hughes, L.E., and G.A. O'Donovan. 1995. Comparison of aspartate transcarbamoylases from Pseudomonas and Pseudomonas-like organisms, abstr. K-115, p. 556. In Abstracts of the 95th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Hughes, L.E., and G.A. O'Donovan. 1995. Pyrimidine metabolism in Streptomyces griseus. Research Appreciation Day. University of North Texas Health Science Center - Fort Worth, Fort Worth, TX.

Hughes, L.E., Linscott, A.J., Beck, D.E., and G.A. O'Donovan. 1994. Pyrimidine metabolism in Streptomyces, abstr. K-43, p. 283. In Abstracts of the 94th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Beck, D.E., Hughes, L.E., Campbell, A.L., and G.A. O'Donovan. 1993. Characterization of pyrimidine salvage pathways by HPLC, abstr. K-74, p. 273. In Abstracts of the 93rd General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

Hughes, L.E., Linscott, A.J., Campbell, A.L., and G.A. O'Donovan. 1993. Pyrimidine metabolism in Streptomyces. Spring Meeting. Texas Branch - ASM, Junction, TX.

O'Donovan, G.A., Schurr, M., Vickrey, J., Campbell, A., Ruley, J., Hughes, L., and D.E. Beck. 1992. Pyrimidine metabolism in Pseudomonas. Lost Pines Molecular Biology Conference. University of Texas M.D. Anderson Cancer Center, Smithville, TX.

Beck, D.E., Campbell, A.L., Hughes, L.E., and G.A. O'Donovan. 1992. Creation of pathways of pyrimidine metabolism not normally present in Pseudomonas. FEBS Meeting. Dublin, Ireland.

Beck, D.E., Hughes, L.E., Campbell, A.L., and G.A. O'Donovan. 1992. Construction of pathways of thymidine metabolism not normally present in Pseudomonas, abstr. K-142, p. 280. In Abstracts of the 92nd General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, DC.

AWARDS, ACCOMPLISHMENTS, AND GRANTS

Attended the NASA Summer School for Planetary Sciences at the Jet Propulsion Laboratory, Pasadena, CA (1998).

Sustaining Member Student Travel Grant, American Society for Microbiology (1998).

Graduate Student Travel Grant, UNT Toulouse School of Graduate Studies (1998).

Outstanding Teaching Assistant Award, UNT Department of Biological Sciences (1997).

Awarded Grant-in-Aid of Research from Sigma Xi, The Scientific Research Society (1996).

Successful Completion of the Microbial Diversity Summer Course at the Marine Biological Laboratory (MBL), Woods Hole, MA (1993).

Received NASA Planetary Biology Internship and Bernard Davis Scholarship to attend MBL summer course (1993).

Texans Scholarship for Doctoral Students, University of North Texas (1993-95).

Who's Who Among Students in American Universities & Colleges (1994).

ACTIVITIES

Sigma Xi, The Scientific Research Society

American Society for Microbiology

UNT Graduate Student Council

Biological Sciences Graduate Resources Committee

REFERENCES